fibroblast growth medium 3 (PromoCell)

PromoCell is a verified supplier

PromoCell manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

PromoCell fibroblast growth medium 3
Fibroblast Growth Medium 3, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/growth+medium/Fibroblast+Growth+Medium+3/custom%40c-23025%4042681974
Average 96 stars, based on 99 article reviews

fibroblast growth medium 3 - by Bioz Stars, 2026-09

96/100 stars

Images

Purification:

Article Title: A tumor metabolism-angiogenesis-immune axis governs immunotherapy responses
Article Snippet: Viable cells were enriched by a 40% Percoll gradient, and the EC fraction was subsequently isolated using CD31 MicroBeads (Miltenyi) according to the manufacturer’s protocol. .. Purified ECs were seeded on XF96 Seahorse plates (Agilent) pre-coated with collagen (10 μg/mL, O.N., 4 °C) at 2×10 4 cells/well and allowed to adhere overnight in endothelial growth medium (PromoCell). mrVEGF-A (PeproTech) was added at 10 ng/ml and cultures were incubated for ∼18hrs. ..

Article Title: Integrated transcriptome and proteome analyses unveil cytoskeletal alterations in an endothelial model of monogenic diabetes.
Article Snippet: On day four, the medium was changed to endothelial cell basal medium (EBM, PromoCell) with 50 ng/ml vascular endothelial growth factor (VEGF, StemCell Technology) and 10 ng/ml of basic fibroblast growth factor (bFGF, StemCell Technology). .. On day 7, differentiated hiPSC-ECs were purified by MoFlo cell sorter based on the CD31 marker and cultured to the first passage on fibronectin-coated wells in endothelial growth medium (EGM MV2, PromoCell) with additional supplementation of 15 ng/ml VEGF (complete medium). ..

Article Title: Integrated transcriptome and proteome analyses unveil cytoskeletal alterations in an endothelial model of monogenic diabetes
Article Snippet: On day four, the medium was changed to endothelial cell basal medium (EBM, PromoCell) with 50 ng/ml vascular endothelial growth factor (VEGF, StemCell Technology) and 10 ng/ml of basic fibroblast growth factor (bFGF, StemCell Technology). .. On day 7, differentiated hiPSC-ECs were purified by MoFlo cell sorter based on the CD31 marker and cultured to the first passage on fibronectin-coated wells in endothelial growth medium (EGM MV2, PromoCell) with additional supplementation of 15 ng/ml VEGF (complete medium). ..

Incubation:

Construct:

Article Title: CRISPR-Cas9-Mediated Upregulation of Utrophin Ameliorates Duchenne Muscular Dystrophy
Article Snippet: .. Following polymerization, constructs were cultured at 37°C in 5% CO2 in 511 growth medium (PromoCell, C-23060) supplemented with 33 μg/μL aprotinin (Sigma, A3428) to 512 inhibit fibrin degradation. .. To induce myogenic differentiation, muscles were transferred to 513 differentiation medium (PromoCell, C-23061) supplemented with aprotinin 48 h after 514 polymerization.

Cell Culture:

Article Title: VE-cadherin interaction proteomics identifies ARVCF as stabilizer of endothelial adherens junctions
Article Snippet: .. Pooled human umbilical vein endothelial cells (HUVECs; mix of female and male donors, P1052, #C2519A, Lonza) and human arterial endothelial cells (HAECs; male donor, #CC2535, Lonza) were cultured until at most passage 8 in endothelial growth medium supplemented with endothelial growth factor-FCS mix (EGM2; #C-22211 + #C-39216, Promocell), 100 U/mL penicillin, and 100 μg/mL streptomycin (#15140122, Gibco) on fibronectin (FN) coated surfaces. ..

Article Title: Micheliolide ameliorates colon cancer cachexia by modulating gut microbiota-immune signaling via Phocaeicola vulgatus enrichment.
Article Snippet: Cells were maintained in Skeletal Muscle Cell Growth Medium (Cat. No. C-23060; basal medium supplemented with Premixed Supplement, PromoCell) according to the manufacturer's instructions. .. For differentiation, the growth medium was replaced with Skeletal Muscle Differentiation Medium (Cat. No. C-23061; basal medium supplemented with Premixed Supplement, PromoCell), and cells were cultured under standard conditions (37 °C, 5% CO2) until myotube formation was achieved. .. Differentiated SkMCs were subsequently treated with CM derived from CT26 or LLC cells in the presence or absence of MCL for 24 h. Similarly, human skeletal myoblasts (HSkMs) obtained from Gibco (Thermo Fisher Scientific) were cultured and differentiated using the differentiation medium according to the manufacturer’s protocol.

Marker:

Recombinant:

Article Title: CD146 + interstitial cells contribute to the dystrophic skeletal muscle phenotype in vitro
Article Snippet: .. The growth medium was replaced with one of the following media: (1) skeletal muscle differentiation medium (C-23061, Promocell), containing 5% FBS, 1% penicillin-streptomycim, 50 mg/ml fetuin, 10 ng/ml epidermal growth factor (EGF), 1 ng/ml fibroblast growth factor (FGF), 10 ug/ml insulin and 0,4 ug/ml dexamethasone; (2) mesenchymal stem cell adipogenic differentiation medium 2 (pre-designed, ready-to-use; C-28016, Promocell); (3) fibrogenic differentiation medium containing DMEM (glucose 4,5 g/l) supplemented with 15% FBS, 1% penicillin/streptomycin and 10 ng/ml recombinant TGFβ (100 − 21; ThermoFisher). ..

Review